Protein function is determined not only by amino acid sequence but also by a wide range of precisely regulated post-translational modifications. Acylation, an important category of these modifications, participates extensively in metabolic homeostasis, epigenetic regulation, protein localization, and essential signaling processes. With the rapid advancement of research on metabolic reprogramming and epigenetic mechanisms, acylation has become an important focus in both life science research and drug discovery. Acylation Quantitative Proteomics enables researchers to systematically compare acylation dynamics under different biological conditions, providing valuable insights into the molecular basis of physiological and pathological processes. These analyses also support the discovery of disease biomarkers and potential therapeutic targets.
Detecting and quantifying protein acylation remains technically challenging because acylated peptides typically exhibit low abundance, high dynamic variability, and diverse modification types. Conventional analytical methods often fail to achieve comprehensive and accurate measurement. Advances in high-resolution mass spectrometry, together with specialized bioinformatics pipelines, are helping to overcome these limitations. MtoZ Biolabs leverages high-resolution mass spectrometry platforms and an established data analysis framework to provide Acylation Quantitative Proteomics analysis services that support high-quality scientific research.
1. What is Acylation Quantitative Proteomics?
Protein acylation is catalyzed by specific enzymes that transfer acyl groups such as acetyl, propionyl, or succinyl from acyl-CoA onto protein residues. Common modification sites include lysine side chains and the protein N terminus. As the fields of metabolism and epigenetics continue to grow, acylation is increasingly recognized as a crucial molecular link between cellular metabolic states and functional regulation.
Acylation Quantitative Proteomics integrates high-resolution mass spectrometry with multiple quantification strategies. These include isotopic labeling techniques such as iTRAQ and TMT, SILAC metabolic labeling, label-free quantification, and DIA-based methods that support large-scale comparison across experimental conditions. For targeted confirmation of specific proteins or modification sites, PRM and MRM offer precise and accurate quantification capabilities.
This systematic analytical approach provides researchers with a comprehensive landscape of acylation events and supports detailed investigation of modification dynamics, enzymatic regulation, and metabolic associations.
Workflow of Mass Spectrometry-Based Acylation Quantitative Proteomics
High-quality acylation analysis requires rigorous sample handling, carefully controlled enrichment procedures, and specialized data interpretation. A typical workflow includes the following steps:
1. Sample Preparation
Deacylase inhibitors are added during sample collection to preserve modification states and prevent artificial alterations. Protein concentration is measured using BCA or comparable assays to ensure consistent sample quality.
2. Enzymatic Digestion
Proteins are digested using trypsin or other sequence-specific proteases to generate peptides suitable for mass spectrometry. Optimized digestion improves peptide ionization efficiency and increases the likelihood of identifying acylation sites.
3. Enrichment of Acylated Peptides
Because acylated peptides are present at very low abundance, enrichment is essential for improving detection depth. Antibody-based enrichment and chemically selective affinity materials are commonly applied. Enrichment strategies can be tailored for different acylation types such as acetylation, succinylation, or crotonylation.
4. Liquid Chromatography Separation
Peptides are separated using high-performance liquid chromatography or nanoLC to improve ionization efficiency and reduce signal suppression. When combined with high-resolution mass spectrometry, nanoLC allows high-precision peptide separation.
5. Mass Spectrometry Analysis
Peptides are ionized by electrospray ionization and analyzed using high-resolution MS1 for accurate mass measurement and MS2 for peptide sequencing and site localization. Orbitrap-based platforms offer the resolving power necessary to differentiate ions with minimal mass differences.
6. Data Analysis and Interpretation
Large-scale mass spectrometry data are processed using a cloud-based bioinformatics system. Analytical steps include spectral interpretation, peptide identification, acylation site localization through FASTA searches, site confidence scoring with FDR control, quantitative comparison across samples, and pathway analysis using GO, KEGG, and Reactome.
Application Prospects of Acylation Quantitative Proteomics
Acylation research contributes to a wide range of scientific and biomedical fields:
1. Basic Biology
Investigating metabolic regulation, epigenetic control, and signaling mechanisms.
2. Disease Research
Identifying abnormal acylation patterns to support disease diagnosis, prognosis, and mechanistic studies.
3. Drug Discovery and Mechanism Evaluation
Under defined experimental conditions, acylation changes can reflect molecular responses to drug treatment and offer valuable reference information for therapeutic development.
With continued advancements in single-cell omics, multi-omics integration, and spatial biology, Acylation Quantitative Proteomics will play an increasingly important role in precision medicine and systems biology.
Advantages of MtoZ Biolabs in Acylation Quantitative Proteomic Analysis
MtoZ Biolabs is a leading mass spectrometry-based CRO specializing in proteomics, metabolomics, biopharmaceutical characterization, and multi-omics integrated analysis. The company utilizes high-resolution mass spectrometry platforms, stable chromatography systems, and well-controlled analytical workflows to support acylation quantitative proteomic research.
1. Advanced Analytical Platforms
MtoZ Biolabs' advanced high-resolution mass-spectrometry platforms ensure reliable, fast, and highly accurate analysis, including the Orbitrap Fusion Lumos, Exploris series, and high-performance liquid chromatography systems.
2. High Data Quality
Deep data coverage and strict data-quality control are maintained. The AI-driven bioinformatics platform integrates all omics analysis data to provide comprehensive reports for clients. The cloud data-analysis system achieves joint interpretation of Acylation Quantitative Proteomics and multi-omics data.
3. Professional Scientific Team
The technical team of MtoZ Biolabs has many years of experience in omics analysis and bioinformatics interpretation and supports customized solutions throughout the entire workflow.
4. Transparent Pricing and One-Stop Service
MtoZ Biolabs offers transparent pricing with no hidden or additional fees. We provide customized Acylation Quantitative Proteomics solutions flexibly adapted to different research needs.
Conclusion
Acylation Quantitative Proteomics offers a powerful approach for uncovering regulatory mechanisms involving protein acylation. Through advanced instrumentation and well-established analytical workflows, MtoZ Biolabs continues to support academic institutions, biotechnology companies, and research teams in achieving high-quality acylation proteomics results. Researchers are welcome to contact MtoZ Biolabs for customized acylation quantitative proteomics solutions
Media Contact
Name: Prime Jones
Company: MtoZ Biolabs
Email: [email protected]
Phone: +1-857-362-9535
Address: 155 Federal Street, Suite 700, Boston, MA 02110, USA
Country: United States
Website: https://www.mtoz-biolabs.com
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